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qPCRBIO SyGreen Mix Separate-ROX

Extremely sensitive. Highly specific. Exceptionally fast. Our SyGreen qPCR master mix utilizes the latest advances in polymerase technology and buffer chemistry to provide outstanding results on any DNA template including genomic, cDNA and viral sequences with the greatest efficiency. This all-in-one master mix requires little to no optimization. The 2x master mix uses an antibody-mediated hot start system that allows for a quick 2 minute activation step. This proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity.

The qPCRBIO SyGreen Mix utilizes a non-PCR inhibiting intercalating dye. Extremely low copy number targets can be detected specifically with the highest efficiency. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions on any real-time PCR cycler. Click on the Instrument Compatibility Guide below to find out which ROX level is compatible with your qPCR instrument.

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Technical Parameters

5 x 1ml Mix & 1 x 200ul ROX
500 x 20ul Rxns/Unit
Brand: PCR Biosystems

  • Uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes
  • Extremely fast extension rate produces earliest Ct values
  • Advanced polymerase technology and buffer chemistry provides highly sensitive and reproducible real-time PCR
  • Universally compatible on all real-time PCR cyclers in both standard and fast cycling conditions
  • Robust, antibody-mediated hot start taq with only a 2 minute activation step
  • Amplification from complex templates including GC-rich sequences and low copy number targets
  • Also available as a blue mix (Cat #: 17-507) with non-reactive blue dye for easy visualization during pipetting